Improving Salmonella isolation

Typical Salmonella isolation typically takes 5-7 days and includes two overnight broth cultures: a non-selective pre-enrichment that allows resuscitation of injured Salmonella, followed by selective enrichment that limits the growth of background microflora. By combining these steps, we have developed protocols that reduce Salmonella isolation by a day.

Previous studies funded by USPE have focused on different sampling approaches such as drip line sampling as an alternative to carcass rinses or whole carcass enrichments as an alternative to carcass rinses. 

Our group and others have observed variability in how Salmonella serovars respond to growth in different media. This can have large impacts on which serovars are isolated when a particular growth medium (e.g., selective enrichment broth) is used.

Relevant papers:

Gorski et al. (2024) Growth assessment of Salmonella enterica multi-serovar populations in poultry rinsates with commonly used enrichment and plating media. Food microbiology. 119:104431.
Rasamsetti et al. (2021) Selective pre-enrichment method to lessen time needed to recover Salmonella from commercial poultry processing samples. Food Microbiology. 99:103818.
Cox et al. (2019) Population analyses reveal preenrichment method and selective enrichment media affect Salmonella serovar detected on broiler carcassess. Journal for Food Protection. 82(10):1688-1696.
Rasamsetti et al. (2022) Assessing Salmonella prevalence and complexity through processing using different culture methods. Poultry Science. 101(7):101949.
Shariat et al. (2022) Animal feed contains diverse populations of Salmonella. Journal of Applied Microbiology. 132(6):4476-4485.
Obe et al. (2021) Comparison of selective enrichment and plating media for Salmonella isolation from broiler carcasses. Journal of Food Safety. 41(6):e12928.